Rapid Determination of Dehydroacetic Acid Content in Chestnuts Using Food Safety Detector
[Objective]To establish an analytical method for rapid determination of dehydroacetic acid(DHA)content in chestnuts using food safety detector.[Method]A food safety detector with a wavelength of 410 nm was used to measure the concentration of the standard solution and draw a standard curve.After extraction with acetone and methanol,the chestnuts reacted with detection reagents,and the reaction solution was automatically calculated and the concentration of deoxyacetic acid was obtained by putting it into the instrument.[Result]Dehydroacetic acid in the tested substance could be well extracted and separated,and the blank solvent had no interference;the standard solution concentration had a good linear relationship between 0-2.5 g/kg(R2=0.999 8),the detection limit and quantification limit of the method were 0.001 4 and 0.004 4 g/kg,respectively.The dehydroacetic acid content in six processed chestnut samples was 0.156,0.321,0.254,0.450,0.285 and 0.389 g/kg,respectively,with relative standard deviation(RSD)ranging from 1.5％to 2.4％.The average recovery rate of blank samples at three different spiking amounts of 0.2,0.6 and 1.0 g/kg ranged from 100.1％to 101.7％,with RSD ranging from 0.9％to 2.9％;there was no significant difference in the test results between this method and the national standard method.[Conclusion]This method has the advantages of simple extraction process,simple operation,short time,high cost-effectiveness,high recovery rate,accurate and reliable results,and good re-producibility,making it suitable for rapid primary screening detection of batch samples.
万方数据
维普
