摘要
为探究外源贝莱斯芽孢杆菌(B.v.)对玉米种芽萌发、幼苗生长及光合系统的调控作用,以郑单 958 和京农科 728 为供试材料接种B.v..结果表明:与CK相比,外源施加B.v.能显著促进玉米种芽萌发,有效提升幼苗株高、叶面积、鲜重、干重与根冠比;与芽孢杆菌菌剂相比,B.v.处理在促进玉米种芽萌发方面具有显著优势,株高、叶面积、干重、鲜重等农艺性状有关指标也与芽孢杆菌菌剂无显著差异.与CK相比,外源B.v.处理下的玉米幼苗Pn、Tr、Gs、WUE显著提升,Ci 较CK显著下降;B.v.处理下的Pn、Tr 和Gs 与芽孢杆菌菌剂处理无显著性差异,但在Ci 利用效能方面显著弱于芽孢杆菌菌剂.与CK相比,外源施加B.v.有助于提升玉米幼苗叶片中叶绿素含量并改善荧光特性,显著提升叶绿素a、叶绿素b、叶绿素a/b、总叶绿素含量、Fm、Fv/Fm、Fv/Fo 和qP,显著降低Fo 和NPQ;B.v.处理下的叶片各叶绿素含量及总含量均显著高于芽孢杆菌菌剂处理,荧光参数整体与芽孢杆菌菌剂无显著差异,部分指标甚至优于芽孢杆菌菌剂处理.因此,贝莱斯芽孢杆菌在玉米上有着良好开发前景和推广应用价值,具有进一步开发成新型菌剂的潜力.
Abstract
To investigate the regulatory effects of exogenous Bacillus velezensis(B.v.)on seed germination,seedling growth and photosynthetic system of maize,Zhengdan 958 and Jingnongke 728 were used as test materials for inoculation with B.v..the results showed that:Compared with CK,exogenous application of B.v.significantly promoted maize seed shoot germination and effectively increased seedling height,leaf are-a,fresh weight,dry weight and root to crown ratio.Compared with Bacillus bacteria agent,B.v.treatment had a significant advantage in pro-moting maize seed germination,and the indexes related to agronomic traits such as plant height,leaf area,dry weight and fresh weight were also not significantly different from those of Bacillus bacteria agent.Compared to CK,maize seedlings under exogenous B.v.treatment showed significantly higher Pn,Tr,Gs and WUE and significantly lower Ci compared to CK.Pn,Tr and Gs under B.v.treatment were not significantly different from Bacillus bacteria agent treatment,but significantly weaker than Bacillus mycoflora in terms of Ci utilization efficiency.Compared with CK,exogenous application of B.v.helped to enhance chlorophyll content and improve fluorescence characteristics in maize seedling leav-es:significantly enhanced chlorophyll a,chlorophyll b,chlorophyll a/b,the total chlorophyll content,Fm,Fv/Fm,Fv/Fo and qP,and signifi-cantly reduced Fo and NPQ.The chlorophyll content and total content of leaves under B.v.treatment were significantly higher than those under Bacillus bacteria agent treatment,and the fluorescence parameters were not significantly different from Bacillus bacteria agent treatment over-all,and some indexes were even better than Bacillus bacteria agent treatment.Therefore,Bacillus velezensis has a good development prospect and application value on corn,and has the potential to be further developed into a new type of bacterial agent.
基金项目
国家重点研发计划(2022YFD2300805)
山东省玉米产业技术体系建设项目(SDAIT-02-11)
山东省技术创新引导计划(2018YFD0300605)
山东省技术创新引导计划(2020LYXZ032)
山东省技术创新引导计划(2021LYXZ004)
维普
万方数据