Effects of Substituting Chemical Fertilizer with Organic Fertilizer Nitrogen on Quality of Greenhouse Spinach,Soil Fertility and Rhi-zosphere Microbial Community
Effects of substituting chemical nitrogen fertilizer with organic nitrogen fertilizer on greenhouse spinach yield,quality,soil fertility and rhizosphere bacterial community were studied.The experiment adopted an equal nitrogen design with six treatments,i.e.,0(CF),20% (OM20),40% (OM40),60% (OM60),80% (OM80),and 100% (OM100)of chemical nitrogen being replaced respectively.The results showed that 20% of chemical nitrogen replacement with organic fertilizer was most conducive to improving the yield and quality of spinach,with fresh and dry weight,vitamin C,and soluble sugar significantly higher than that of CF.In all treatments,adding organic fertilizer could significantly increase the soil pH,organic matter,nitrogen,available phosphorus,and available potassium content,and significantly reduce the bulk density.When the proportion of organic nitrogen fertilizers was 20%-80%,the content of alkaline nitrogen was significantly lower than CF.Applying organic fertilizer could significantly increase the α diversity of soil bacteria and reduce β diversity.Spinach organic acid content and soil alkaline nitrogen content significantly contributed to β diversity,which was 12.80% and-4.71% respectively.Application of organic fertilizer can improve the relative abundance of beneficial bacteria such as aerobic and stress tolerant,but it might also increase the rel-ative abundance of potential pathogenic bacteria.In summary,the replacement of chemical nitrogen fertilizer with organic nitrogen fertilizers can improve the quality of spinach and improve soil fertility and resistance.
Organic fertilizerSpinachSubstituting chemical fertilizerSoil fertilityBacterial community