安徽农业科学2024,Vol.52Issue(7) :169-173.DOI:10.3969/j.issn.0517-6611.2024.07.040

体外模拟消化对鲟鱼头硒螯合肽结构及抗氧化活性的影响

Effects of in vitro Simulated Digestion on the Structure and Antioxidant Activity of Sturgeon Head Peptides-selenium

贾娇 吴汶飞
安徽农业科学2024,Vol.52Issue(7) :169-173.DOI:10.3969/j.issn.0517-6611.2024.07.040

体外模拟消化对鲟鱼头硒螯合肽结构及抗氧化活性的影响

Effects of in vitro Simulated Digestion on the Structure and Antioxidant Activity of Sturgeon Head Peptides-selenium

贾娇 1吴汶飞1
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作者信息

  • 1. 大连工业大学食品学院,辽宁大连 116034;大连工业大学国家海洋食品工程技术研究中心,辽宁大连 116034
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摘要

为了明确鲟鱼头蛋白肽和鲟鱼头硒螯合肽的抗氧化活性差异,体外模拟了口腔及胃肠道消化处理过程中鲟鱼头蛋白肽和鲟鱼头硒螯合肽的结构及抗氧化活性变化.结果表明:鲟鱼头硒螯合肽的紫外光谱吸收峰在体外口腔胃肠模拟消化过程中比鲟鱼头蛋白肽更加稳定.体外模拟消化导致鲟鱼头蛋白肽和鲟鱼头硒螯合肽的圆二色谱吸收峰强度显著降低.粒径分布结果也表明:鲟鱼头硒螯合肽在消化过程中具有更好的溶解性.ABTS、DPPH以及OH自由基清除活性结果显示,在体外模拟消化后,鲟鱼头蛋白肽和鲟鱼头硒螯合肽的自由基清除活性进一步增强,且鲟鱼头硒螯合肽的自由基清除能力最强.

Abstract

In order to clarify the difference in antioxidant activity of sturgeon head peptides and sturgeon head peptides-selenium,the structure and antioxidant activity changes of sturgeon head peptides and sturgeon head peptides-selenium during oral and gastrointestinal digestion were simulated in vitro.The results showed that the UV absorption peak of sturgeon head peptides-selenium is more stable than that of sturgeon head peptides during in vitro simulated oral gastrointestinal digestion.in vitro simulated digestion resulted in a significant decrease in the circular di-chroism absorption peak intensity of sturgeon head peptides and sturgeon head peptides-selenium.The particle size distribution results also in-dicate that sturgeon head peptides-selenium has better solubility during digestion.The results of ABTS·+,DPPH·and·OH scavenging ac-tivity showed that after simulated digestion in vitro,the free radical scavenging activities of sturgeon head peptides and sturgeon head peptides-selenium were further enhanced,and sturgeon head selenium-chelating peptide had the strongest free radical scavenging ability.

关键词

鲟鱼头蛋白肽/硒螯合肽/结构特性/体外模拟/抗氧化活性

Key words

Sturgeon head peptides/Selenium-chelating peptides/Structural characteristics/in vitro simulation/Antioxidant activity

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基金项目

辽宁省博士科研启动基金(2019-BS-017)

出版年

2024
安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
参考文献量28
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