Effects of Acid Treatment and Enzymatic Hydrolysis on Reducing Sugar and Cellulose Degradation of Pleurotus eryngii Residue
In order to explore the effects of acid treatment (acid concentration,time and temperature),enzyme preparations (cellulase,xyla-nase,β-glucanase) on Pleurotus eryngii fungus residue.The experiment used the mushroom residue of Pleurotus eryngii collected in Hebei Prov-ince as the raw material,total reducing sugar content as the research index,the factors affecting the acid treatment of P.eryngii residue were an-alyzed by principal component analysis.At the same time,the effects of enzyme preparation on the reduction of neutral detergent cellulose and acid detergent cellulose were analyzed by orthogonal test.At 40 °C,2％ and 3％ acid concentrations would degrade the content of reducing sug-ar with time.At 60 °C,the increase of acid concentration could slow down the degradation of total reducing sugar.At 80 ℃,1％ and 2％ acid concentrations could promote the degradation of polysaccharides and increase the content of total reducing sugar. According to the principal component analysis,temperature was the most important factor affecting the content of reducing sugar of P.eryngii slag.The orthogonal test re-sults showed that the degradation of xylanase and cellulase were the main factors affecting the reduced amount of neutral detergent cellulose and acid detergent cellulose respectively.The optimal enzymolysis formula was cellulase 85 U/mL,xylanase 220 U/mL and glucanase 40 U/mL. These results indicated that the bacterial residue after acid and enzyme treatment has nutritional value as a ruminant feed.
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维普
