In order to fully develop the utilization value of medicinal and edible materials of fresh Dendrobium officinale and prepared rehman-nia root, enrich the types of yogurt. Using whole milk powder, fresh Dendrobium officinale, prepared rehmannia root and xylitol as raw materi-als, the production process of Dendrobium officinale prepared rehmannia root yogurt was studied. With the sensory quality as the evaluation in-dex, the effects of the addition of Dendrobium officinale extract, the addition of prepared rehmannia root extract, the addition of xylitol, the ad-dition of milk powder, the addition of strain and the fermentation time on the quality of yogurt with Dendrobium officinale and prepared rehman-nia root were investigated by single factor test and orthogonal experiment to determine the optimum production conditions. The results showed that the optimum formula and process were as follows:the addition Dendrobium officinale extract was 5%, the addition of prepared rehmannia root extract was 6%, the addition of xylitol was 11%, the addition of milk powder was 17%, the addition of strain was 0.32%, the fermenta-tion temperature was 42℃, and the fermentation time was 6 h. Solidified yogurt with the Dendrobium officinale and prepared rehmannia root with good quality was obtained under the conditions, showing a stable and uniform light yellow, moderate sweet and sour, smooth taste, deli-cate texture, with the special grass flavor of Dendrobium officinale. Its physical and chemical indexes and microbial indexes all meet the re-quirements of national standards.