Study on the Process of Separating and Purifying Total Flavonoids from Licorice Waste Residue Using Macroporous Resin
To develop a process for separating and purifying total flavonoids from licorice waste residue using macroporous resin. [ Method] Comparing the adsorption capacity and desorption rate of D101B, AB-8 and DM-130 for total flavonoids to screen the optimal resin. Single factor experiments were used to screen the sample concentration, sample flow rate, sample volume, 20％ ethanol elution volume, eluent concentration and eluent volume during the dynamic adsorption process.[Result]AB-8 macroporous resin had the best separation and purifica-tion effect for total flavonoids in licorice waste residue. The sample concentration was 2.345-3.126 mg/mL, the sample flow rate was 1.0-1.5 mL/min, and the sample volume was 64 mL,20％ ethanol elution volume was 5 BV, and 80％ ethanol elution volume was 4 BV. After this purification process, the total flavonoid concentration increased from 15.63％ to 65.68％.[Conclusion]This method is suitable for the prelimi-nary separation and purification of total flavonoids from licorice waste residue.
Macroporous adsorption resinSeparation and purificationLicorice residueTotal flavonoidsProcess
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