To develop a process for separating and purifying total flavonoids from licorice waste residue using macroporous resin. [ Method] Comparing the adsorption capacity and desorption rate of D101B, AB-8 and DM-130 for total flavonoids to screen the optimal resin. Single factor experiments were used to screen the sample concentration, sample flow rate, sample volume, 20％ ethanol elution volume, eluent concentration and eluent volume during the dynamic adsorption process.[Result]AB-8 macroporous resin had the best separation and purifica-tion effect for total flavonoids in licorice waste residue. The sample concentration was 2.345-3.126 mg/mL, the sample flow rate was 1.0-1.5 mL/min, and the sample volume was 64 mL,20％ ethanol elution volume was 5 BV, and 80％ ethanol elution volume was 4 BV. After this purification process, the total flavonoid concentration increased from 15.63％ to 65.68％.[Conclusion]This method is suitable for the prelimi-nary separation and purification of total flavonoids from licorice waste residue.
关键词
大孔吸附树脂/分离纯化/甘草废渣/总黄酮/工艺
Key words
Macroporous adsorption resin/Separation and purification/Licorice residue/Total flavonoids/Process
维普
万方数据