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红茶菌发酵液宏基因组分析

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对3个发酵批次的红茶菌发酵液进行宏基因组序列分析,采用数据库进行比对,发现红茶菌发酵液中存在短乳杆菌、植物乳杆菌、食窦魏斯氏菌、发酵乳杆菌、戊糖乳杆菌、融合魏斯氏菌、罗伊氏乳杆菌、柠檬明串珠菌、类植物乳杆、副干酪乳杆菌、乳酸片球菌等具有益生功能的细菌.宏基因组注释到的功能基因共有76512个,其中与代谢过程注释到的功能基因数最多,且这些代谢途径上注释得到的菌株多数是益生菌株,主要参与了与碳水化合物代谢相关的三羧酸循环、柠檬酸循环、磷酸戊糖途径、糖酵解等;与氨基酸代谢相关主要有丙氨酸、天冬氨酸和谷氨酸代谢途径、甘氨酸、丝氨酸和苏氨酸等代谢途径.
Metagenomic Analysis of Kombucha Fermentation Broth
The metagenomic sequence analysis of the fermentation broth of three fermentation batches of kombucha was carried out,and the database was used for comparison. It was found that there were mainly Lactobacillus brevis,Lactobacillus plantarum,Weisseria sinusosus,Lacto-bacillus fermentatum,Lactobacillus pentosus,Weisseria fused,Lactobacillus reuteri,Nebulococci citronicum,Lactobacillus paracasei,Lococci lac-tate and other bacteria with probiotic function in the fermentation broth of tea fungus. A total of 76512 functional genes were annotated by met-agenomes,among which the most functional genes were annotated by metabolic processes. Most of the strains annotated by these metabolic pathways were probiotic strains,which were mainly involved in the tricarboxylic acid cycle,citric acid cycle,pentose phosphate pathway,gly-colysis and so on related to carbohydrate metabolism. The metabolic pathways of alanine,aspartate and glutamate,glycine,serine and threo-nine are mainly related to amino acid metabolism.

KombuchaProbioticsMetagenomeAnalysis

赵平、刘庆静、杨光、郭莹莹、邹积宏

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黑龙江省绿色食品科学研究院,黑龙江哈尔滨 150028

黑龙江大学,黑龙江哈尔滨 150080

黑龙江省质量监督检测研究院,黑龙江哈尔滨 150028

红茶菌 益生菌 宏基因组 分析

2024

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2024.52(9)