首页|3株内生菌对棉花枯萎病菌拮抗效果研究

3株内生菌对棉花枯萎病菌拮抗效果研究

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[目的]探究3株棉花内生菌对棉花枯萎病菌的拮抗效果.[方法]以棉花枯萎病菌为试材,采用平板对峙法将3株内生菌菌悬液、上清液、菌挥发物分别对峙棉花枯萎病菌研究3株内生菌拮抗效果.[结果]3株内生菌菌悬液对棉花枯萎病菌的拮抗效果为O>T>DG3-1;3株内生菌上清液对棉花枯萎病菌的拮抗效果为T>O>DG3-1;3株内生菌菌挥发物对棉花枯萎病菌拮抗效果为DG3-1>T>O.并对抑制过的枯萎病菌菌丝形态进行显微观察,结果显示,菌丝出现空腔、断裂以及细胞壁部分溶解.重新培养抑制过的枯萎病菌,枯萎病菌可以正常生长,但生长较慢.[结论]3株内生菌对枯萎病菌生长有不同程度的影响.
Study on the Antagonistic Effect of Three Strains of Endophytic Bacteria on Cotton-wilt Fusarium
[Objective]To explore the antagonistic effect of three endophytic bacteria in cotton against Fusarium wilt.[Method]Using the plate confrontation method,three endophytic bacterial suspensions,supernatants,and volatiles were used to study the antagonistic effects of the three endophytic bacteria against the cotton Fusarium wilt pathogen. [Result]The antagonistic effect of the three bacterial suspensions on Fu-sarium oxysporum was O>T>DG3-1. The antagonistic effect of the supernatant of the three bacterial strains on F. oxysporum was T>O>DG3-1; the antagonistic effect of volatiles from three strains of bacteria on F. oxysporum was DG3-1>T>O. Microscopic observation of the morphol-ogy of the mycelium that suppressed F. oxysporum showed cavities,breakage,and partial dissolution of the cell wall. Recreate the inhibited F. oxysporum,which could grow normally but slowly. [Conclusion]Three endophytic bacteria have varying degrees of influence on the growth of Fusarium wilt.

BacillusRhizobiumEndophytic bacteriaCotton Fusarium wiltAntagonism

祖丽胡玛尔·买提喀迪尔、代先兴、李猛、王天舒、闫成才、牛冬冬、郝海婷

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塔里木大学南疆农业有害生物综合治理重点实验室,新疆阿拉尔 843300

南京农业大学植物保护学院,江苏南京210095

芽孢杆菌 根瘤菌 内生菌 棉花枯萎病 拮抗

2024

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2024.52(9)