首页|不同蔬菜种植对虾菜共生系统中微生物群落结构的影响

不同蔬菜种植对虾菜共生系统中微生物群落结构的影响

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[目的]研究蕹菜、甘薯、枸杞对虾菜共生系统中微生物群落结构的影响.[方法]通过高通量测序技术分析种植不同蔬菜的虾菜共生系统中微生物群落组成,并检测该系统中的硝酸盐、磷酸盐指标.[结果]不同虾菜共生系统中水体微生物共同的OTU数目为450种,其中,枸杞组(LM)的OTU数目最多,为852种,且Shannon-Wiener指数和Simpson指数最高;蕹菜组(IF)对硝酸盐、磷酸盐去除率分别达65.70%和67.57%,且与其他组群落结构差异显著(P<0.05).在门水平上,不同系统中水体的优势菌群为变形菌门(Proteobacte-ria)、放线菌门(Actinobacteria)、Patescibacteria;在属水平上,不同系统中的优势菌群为红球菌属(Rhodococcus)、不动杆菌属(Acinetobact-er)、谷氨酸杆菌属(Glutamicibacter).[结论]甘薯和枸杞更有利于维持水环境的稳定,蕹菜对硝酸盐、磷酸盐去除效果更好.
Effects of Different Vegetable Cultivation on the Microbial Community Structure in Shrimp-vegetable Symbiotic System
[Objective] To study the effects of Ipomoea aquatica, Ipomoea batatas and Lycium chinense on the microbial community structure in shrimp-vegetable symbiotic system.[Method]The composition of microbial community in shrimp-vegetable symbiotic system growing different vegetables was analyzed by high-throughput sequencing technique, and the indexes of nitrate and phosphate in the system were detected. [ Re-sult] The number of OTU shared by microorganisms in different shrimp-vegetable symbiotic systems was 450 species,among them, the number of OTU in Lycium chinense group (LM) was the highest (852 species), and the Shannon-Wiener index and Simpson index were the highest. The removal rates of nitrate and phosphate in Ipomoea aquatica (IF) were 65.70% and 67.57%, respectively, and the community structure was significantly different from other groups (P<0.05). At the gate level, the dominant flora in different systems were Proteobacteria, Acti-nobacteria and Patescibacteria.At the genus level,while the dominant flora in different systems were Rhodococcus, Acinetobacter and Glutamici-bacter. [ Conclusion] Ipomoea batatas and Lycium chinense were more beneficial to maintain the stability of water environment, and Ipomoea aquatica was more effective in removing nitrate and phosphate.

Shrimp-vegetable symbiosisVegetable cultivationMicroorganismCommunity structureHigh-throughput sequencing

任思齐、应赵晗、吴昕睿、李明、贺文芳、田磊、徐勇斌、程霄玲、郑善坚

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浙江师范大学生命科 学学院,浙江金华321004

浙江省野生动物生物技术与保护利用重点实验室,浙江金华321004

金华市水产技术推广站,浙江金华321000

虾菜共生 蔬菜种植 微生物 群落结构 高通量测序

金华市重点研发项目浙江省重点研发计划

2021-2-0172020C02014

2024

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2024.52(10)
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