Expression Analysis of GPx and GST Genes in Ruditapes philippinarum Under Heavy Metal Mercury Stress
In order to study the gene expression of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) in Ruditapes philippina-rum under the stress of heavy metal mercury ( Hg) , a single chronic exposure experiment on the indicator organism R. philippinarum was car-ried out. The expression of two genes in visceral mass and gill of R. philippinarum was detected at 0,12 h,24 h and 2, 3, 4, 5, 6, 7 and 8 d. The results showed that the expression multiples of GPx and GST in the visceral mass showed a fluctuation trend, and the expression multiples reached the highest ( P<0.05) at 24 and 12 h, respectively. The expression level was the lowest at 6 d( P>0.05) and 3 d ( P<0.05) . The ex-pression multiple of GPx in the gill was the highest at 8 d ( P< 0.05) , and the first peak appeared at 5 d. The expression amount of GST was the highest at 5 d ( P<0.05) . It showed that mercury exposure could induce the expression of GPx and GST in different degrees in the short term,but it had obvious inhibitory effect with the extension of time. This study provided the theoretical basis for revealing the toxicity of heavy metal mercury to R. philippinarum.
Heavy metal mercuryR. philippinarumGlutathione peroxidaseGlutathione-S-transferase
万方数据
维普
