基于BSA-seq技术挖掘番茄耐裂果候选基因

Extraction of Tomato Cracking Tolerance Candidate Genes Based on BSA-seq Technique

冯汝龙 ¹王彦刚 ¹常晨晨 ¹景涛 ¹冯锡鸿 ¹申太荣 ¹董建力²

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作者信息

1. 宁夏中青农业科技有限公司,宁夏银川750001
2. 宁夏中青农业科技有限公司,宁夏银川750001;宁夏农林科学院农业生物技术研究中心,宁夏银川750002
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摘要

[目的]明确番茄耐裂基因在染色体上的位置.[方法]以耐裂果番茄14FP5和易裂果番茄14FP26为试材,将二者杂交得到F1代,F1自交得到F2,分别构建2个极端性状的DNA混合池,采用BSA-seq方法进行定位.[结果]利用BSA-seq对F2群体的2个极端混池进行基因组重测序分析,将番茄耐裂基因定位在5号染色体上,位于34140000～38120000 pb,区间大小为3.98 Mb,候选区间内共注释到38个基因,其中非同义突变基因4个.[结论]该研究结果可为番茄耐裂基因的精细定位提供数据支撑,为番茄耐裂分子育种奠定基础.

Abstract

[Objective] To determine the position of tomato crack tolerance gene on chromosome.[Method]Tomato 14FP5 which is resistant to cracking and tomato 14FP26 which is easy to crack are used as test materials,cross the two to get F1 generation, F1 self-cross to get F2 ,DNA mixing pools with two extreme traits were constructed respectively,BSA-seq method was used to locate the location.[Result]BSA-seq was used for genome resequencing analysis of 2 extreme mixed pools of F2 population.The tomato anti-cracking gene was initially located on chromosome 5,located between 34140000 and 38120000 pb,the interval size is 3.98 Mb,a total of 38 genes were annotated in the candidate region,in-cluding 4 non-synonymous mutant genes.[Conclusion]The results provide data support for the fine localization of tomato crack tolerance genes, and lay a foundation for tomato crack tolerance molecular breeding.

关键词

番茄/耐裂性/BSA-seq/基因定位/候选基因

Key words

Tomato/Cracking-resistant/BSA-seq/Assignment of genes/Candidate gene

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基金项目

宁夏回族自治区自然科学基金(2020AAC03508)

宁夏回族自治区重大科技项目(2017DC55)

出版年

2024

安徽农业科学

安徽省农业科学院

安徽农业科学

影响因子：0.413

ISSN：0517-6611

参考文献量4

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