Extraction of Tomato Cracking Tolerance Candidate Genes Based on BSA-seq Technique
[Objective] To determine the position of tomato crack tolerance gene on chromosome.[Method]Tomato 14FP5 which is resistant to cracking and tomato 14FP26 which is easy to crack are used as test materials,cross the two to get F1 generation, F1 self-cross to get F2 ,DNA mixing pools with two extreme traits were constructed respectively,BSA-seq method was used to locate the location.[Result]BSA-seq was used for genome resequencing analysis of 2 extreme mixed pools of F2 population.The tomato anti-cracking gene was initially located on chromosome 5,located between 34140000 and 38120000 pb,the interval size is 3.98 Mb,a total of 38 genes were annotated in the candidate region,in-cluding 4 non-synonymous mutant genes.[Conclusion]The results provide data support for the fine localization of tomato crack tolerance genes, and lay a foundation for tomato crack tolerance molecular breeding.
TomatoCracking-resistantBSA-seqAssignment of genesCandidate gene
万方数据
维普
