Study on Dormancy and Release of Cassia agnes Brenan Seed
In order to explore the dormancy reason and dormancy release method of Cassia agnes seeds,in this research,we mainly studied the biological characteristics and germination inhibitors of C.agnes seeds,hot water,concentrated sulfuric acid and gibberellin (GA3) were used to break the seed dormancy.The results showed that the seed was elliptic,the seed skin was yellowish-brown,smooth and hard,the 1000-seed weight was 378.66 g,the water content was 9.97％,the seed viability was 100％,and the germination potential was great.After soaking the whole seeds for 215 h,the water absorption rate was only 0.57％,which was significantly different from that of seeds broken skin.The methanol extract from the seed coat and seed embryo of C.agnes had significant inhibitory effects on the germination of Chinese cabbage seeds and the growth of seedling roots.The germination rate was higher (53.33％) when the seeds were soaked in 98％ concentrated sulfuric acid for 10 min. Soaking seeds in the water with different temperature until natural cooling had little promoting effect on germination,and the highest germination rate was only 5.56％.The seeds soaked in gibberellin (GA3) can not promote the germination of seeds.The main reasons of C.agnes seeds dor-mancy were the water absorption barrier.During the stratification at four temperature,the soluble sugar content had little change,in the process of stratification at twenty-five temperature,the soluble sugar content first increased and then decreased,while the soluble protein and crude fat contents showed a decrease trend and the rate of decrease at twenty-five temperature stratification was faster,indicating that a large amount of nutrients were needed in the process of seed dormancy removal.
万方数据
维普
