摘要
[目的]明确绿色木霉(Trichoderma viride)真菌的生物学特性及对盐胁迫下玉米幼苗的缓解机制.[方法]对前期筛选出一株绿色木霉菌株进行生物学特性分析,并通过测定绿色木霉对盐胁迫下玉米幼苗株高、根长、地上部分干质量、地下部分干质量、根冠比、叶绿素a含量、叶绿素b含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)等指标的影响,探讨绿色木霉对盐胁迫下玉米幼苗生长及生理指标的影响.[结果]菌株的最适生长温度是25℃;24 h黑暗处理下菌落直径显著高于12 h黑暗+12 h光照、24 h光照2个处理;菌株在pH为5时生长最快.添加不同浓度绿色木霉孢子悬浮液后,盐胁迫下玉米幼苗株高、根长、地下部分及地上部分干质量、叶绿素a和叶绿素b含量、SOD活性、POD活性、CAT活性、PPO活性均呈现逐渐上升的趋势.[结论]研究结果可为提高玉米在盐碱土壤种植效率提供基础资料.
Abstract
[Objective]To clarify the biological characteristics of Trichoderma viride and their mechanisms of alleviating salt stress in corn seedlings.[Method] The biological characteristics of a Trichoderma viride strain screened in the early stage were analyzed,and the effects of Trichoderma viride on corn seedling height,root length,aboveground dry weight,underground dry weight,root crown ratio,chlorophyll a content, chlorophyll b content,SOD,POD,CAT,PPO and other indicators under salt stress were measured,exploring the effects of Trichoderma viride on the growth and physiological indicators of corn seedlings under salt stress.[Result]The optimal growth temperature for the strain was 25 ℃;the requirements for light were not strict,but the colony diameter under 24 hours of darkness treatment was significantly higher than 12 hours of darkness+12 hours of light,and 24 hours of light;the strain grows fastest at pH=5.After adding different concentrations of spore suspension,the plant height,root length,dry weight of underground and aboveground parts,chlorophyll a and chlorophyll b content,SOD activity,POD activity, CAT activity,and PPO activity of corn seedlings showed a gradually increasing trend under salt stress.[Conclusion]The research results can provide basic data for improving the planting efficiency of corn in saline alkali soil.
基金项目
邯郸市科学技术研究与发展计划项目(21212012057)
邯郸市社科联规划课题(2022142)
维普
万方数据