Isolation, Identification and Antibiotic Resistance of Pathogen Isolated from Avian with Multicausal Airsacculitis
In order to investigate the major pathogen of multicausal airsacculitis and its antibiotic resistance in a hennery in Sihui area of Guangdong Province, the morphological identification, 16S rRNA gene sequencing were performed, and drug resistance of these isolates were assessed using MIC. The results showed that the colonies of the first isolate had a typical "poached egg"shape, the second isolate was Gram-negative bacilli and the third isolate was Gram-positive cocci. 16S rRNA gene sequencing analysis results showed that the isolated strains were identified as Mycoplasma gallinarum, Escherichia coli and Staphylococcus aureus, named as SH-MG strain, SH-E strain and SH-S strain, re-spectively. Drug resistance results showed that SH-E strain was resistant to amoxicillin, florfenicol and tiamulin, and SH-S strain was resistant to amoxicillin. We conclude that the multicausal airsacculitis is probably caused by co-infection of drug-resistant Staphylococcus aureus, drug-resistant Escherichia coli and Mycoplasma gallinarum, tiamulin is the first choice for the prevention and treatment of mycoplasma gallinis infec-tion.
Multicausal airsacculitisIsolation and identificationDrug resistanceMycoplasma gallisepticumAvian pathogenic Escherichia coliStaphylococcus aureus
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维普
