Study on DNA Barcoding Identification Methods of Part Species of Latrodectus
Four species of spiders intercepted at domestic ports were studied in this paper. The CO Ⅰ sequences of which were amplified by PCR and compared with the CO Ⅰ sequences of five species recorded in GenBank. And the sequence characteristics were analyzed and the phylogenetic tree was constructed.The results showed that there were significant genetic differences among different species of Latrodectus, and the CO Ⅰ sequence could be used as a DNA barcode for molecular identification of different species of Latrodectus.