Optimization of Extraction Process and Antioxidant Activity of Polyphenols from Artemisia argyi
[Objective]Taking Artemisia argyi leaves as the research object,in order to explore the optimal extraction process and antioxidant activities of polyphenols from Artemisia argyi.[Method]Single-factor test was used to investigate the influence of ethanol concentration,liquid-material ratio and ultrasonic extraction time on the extraction amount of Artemisia argyi polyphenols.On this basis,the extraction process was optimized by Box-Behnken response surface analysis,and the antioxidant activity of polyphenols was evaluated by the ability to remove DPPH free radicals and hydroxyl radicals.[Result]The optimal process was 77％ ethanol concentration,material-liquid ratio 24:1 (mL: g) and the extraction time 53 min.Under the above conditions,the extraction amount of polyphenols was 41.31 mg/g.Antioxidant activity analysis showed that polyphenols had good antioxidant ability. The semi-inhibition concentrations (IC50) of DPPH free radicals and hydroxyl radicals were 0.0504 and 0.0311 mg/mL respectively.[Conclusion]Using ultrasound assisted method,the extraction amount of Artemisia argyi polyphenols reached 41.31 mg/g under the optimal extraction process conditions.The extracted Artemisia argyi polyphenols under these conditions had good antioxidant properties.This method was simple,easy to operate and had a high yield.
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