Study on the Growth-promoting Effect of Rhizobia on Tumorous Stem Mustard
[Objective]To study the infection of Azorhizobium caulinodans in tumorous stem mustard and its effect on plant growth promotion.[Method]The differences in growth promoting effects of rhizobia on different varieties of tumorous stem mustard,the effects of different concen-trations of rhizobia on the cultivated variety'Fuza 2'of tumorous stem mustard and the effects of different inducers on nodulation were ana-lyzed.[Result]The root length and plant height of'Fulza 2'increased significantly,and the growth promotion effect was obvious among the 6 stem mustard varieties infected with nitrogen-fixing rhizobia.The results of different concentrations of Azorhizobium caulinodans showed that the root length and plant height of'Fulza 2'seedlings increased significantly at the concentration of 1.0×108 rhizobium/mL,and the colonization of rhizobium in the root tissues of healthy'Fulza 2'seedlings was observed by fluorescence microscopy.After 3 days of treatment,rhizobium could invade and colonize the roots of'Fulza 2'seedlings.After 15 days of inoculation,high population density was developed in the local tis-sues of stems.Horizontal comparison of the three inducers'nodulation efficiency showed that the three inducers could form nodule in the roots of stem tumor mustard after 30 days,and the number of nodule increased significantly after 60 days.The nodulation efficiency of 2,4-D was the best,up to 93%,which was significantly higher than that of the other two inducers.[Conclusion]This study lays the foundation for better utili-zing the symbiotic system of rhizobia to promote the growth of stem mustard.
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