Study on Vegetative Compatibility of Cytospora pyri from Korla Fragrant Pear in Different Planting Areas
[Objective]To use Nit mutant system to explore the genetic diversity of fragrant pear from different geographical sources in Xin-jiang.[Method]In this study,Nit mutant technology(nitrate-resistant defective mutant)was used for the first time to study the nutritional af-finity of Cytospora pyri,13 strains of Korla pear tree rot from three different growing areas in southern Xinjiang.[Result]115 Nit mutants were cultured in chlorate medium(KPS)from 13 strains,and 41 of them did not mutate.In the identification of mutant stability,33 stable Nit mu-tants and 82 unstable Nit mutants were identified.The phenotype of the resulting Nit mutants was identified as NitD 85%and NitB 15%.[Con-clusion]In this study,two Nit mutants were obtained,indicating that there may be two mutations in the nitrogen assimilation pathway of C.pyri.All isolates tested were able to utilize hypoxanthine,and the ability to use hypoxanthine ruled out the possibility that molybdenum cofactor sites were blocked.
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