不同种植区库尔勒香梨树腐烂病菌Cytospora pyri营养体亲和性研究
Study on Vegetative Compatibility of Cytospora pyri from Korla Fragrant Pear in Different Planting Areas
彭莎莎 1王喆 1沙帅帅 2张怡雯 1闫成才 1王兰1
作者信息
- 1. 塔里木大学农学院/南疆农业有害生物综合治理兵团重点实验室/南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室,新疆阿拉尔 843300
- 2. 喀什大学现代农学院,新疆喀什 844006
- 折叠
摘要
[目的]使用Nit突变体体系,探索来自新疆香梨种植区不同地理来源的腐烂病菌遗传多样性.[方法]首次使用Nit突变株技术(抗氯酸盐的硝酸盐利用缺陷突变体)对来自南疆3个不同种植区的13株库尔勒香梨树腐烂病菌Cytospora pyri的营养体亲和性进行了研究.[结果]13个菌株在含氯酸盐培养基(KPS)培养出115个Nit突变体,其中41个没有发生突变.在突变体稳定性鉴定中,鉴定出33个稳定的Nit突变体,82个不稳定的Nit突变体.产生的Nit突变体的表型85%被鉴定为NitD,15%被鉴定为NitB.[结论]获得2 个Nit突变体,说明C.pyri的氮同化途径可能发生了2种突变.所有被检测的分离株都能够利用次黄嘌呤,使用次黄嘌呤的能力排除了钼辅助因子位点被阻断的可能性.
Abstract
[Objective]To use Nit mutant system to explore the genetic diversity of fragrant pear from different geographical sources in Xin-jiang.[Method]In this study,Nit mutant technology(nitrate-resistant defective mutant)was used for the first time to study the nutritional af-finity of Cytospora pyri,13 strains of Korla pear tree rot from three different growing areas in southern Xinjiang.[Result]115 Nit mutants were cultured in chlorate medium(KPS)from 13 strains,and 41 of them did not mutate.In the identification of mutant stability,33 stable Nit mu-tants and 82 unstable Nit mutants were identified.The phenotype of the resulting Nit mutants was identified as NitD 85%and NitB 15%.[Con-clusion]In this study,two Nit mutants were obtained,indicating that there may be two mutations in the nitrogen assimilation pathway of C.pyri.All isolates tested were able to utilize hypoxanthine,and the ability to use hypoxanthine ruled out the possibility that molybdenum cofactor sites were blocked.
关键词
香梨树腐烂病菌/Cytospora/pyri/Nit突变体Key words
Pear Valsa Canker/Cytospora pyri/Nit mutant引用本文复制引用
基金项目
国家自然科学基金-新疆联合基金重点项目(U1903206)
旱区作物逆境生物学国家重点实验室开放基金(CSBAA2020009)
自治区研究生科研与创新项目(XJ2021G293)
出版年
2024
国家科技期刊平台
NSTL
万方数据