Study on Anti-oxidation and Anti-fatigue Effects of Medicinal and Edible Homologous Beverage for TCM Based on Zebrafish Model
[Objective]To analyze the chemical constituents in 2 medicinal and edible homologous beverage(Xiangfeng Yueyan Drink and Shenqi Guyuan decoction),and evaluate the anti-oxidant and anti-fatigue effects.[Method]UHPLC-TQ-Orbitrap-MS mass spectrometer and reverse phase C18 column were used to analyze the composition of the samples.The oxidative stress model established by aqueous menadione administration method was added with different gradient concentrations(25,50,100 μg/mL)of Xiangfeng Yueyan Drink dilution.Meanwhile,a normal group and a positive drug control group were set up.After treatment for 24 h,the Cell ROX was stained without light,and the fluores-cence intensity of yolk sac was calculated by fluorescence microscope.Select 5-day-old wild type zebrafish and add different gradient concen-trations(25,50,100 μg/mL)of Shenqi Guyuan decoction diluent.A normal control group and a positive drug group were set up at the same time.After treatment for 18 h,the fatigue model of zebrafish was established by aqueous solution of anhydrous sodium sulfite in the administra-tion group,and the total movement distance of zebrafish was analyzed by behavior analyzer.[Result]According to the mass spectrometry data,63 compounds were detected in Xiangfeng Yueyan Drink and 60 compounds were detected in Shenqi Guyuan Drink.Compared with the model group,the fluorescence intensity of zebrafish yolk sac significantly weakened under the intervention of Xiangfeng Yueyan Drink(P＜0.01);compared with the model control group,the total movement distance of zebrafish under the intervention of Shenqi Guyuan Drink was signifi-cantly longer(P＜0.05).[Conclusion]The 2 kinds of medicinal and edible homologous beverage have clear anti-oxidation and anti-fatigue effects respectively.
ZebrafishHomology of medicine and foodLC-MSAnti-oxidationAnti-fatigue
国家科技期刊平台
NSTL
万方数据
