Comparation of the Effectiveness of Powder MS Medium in Eight Brands
[Objective]To compare the effectiveness of 8 brands of powder MS medium,so as to provide a reference basis for selecting medi-um for plants tissue culture.[Method]Using the leaves,petioles,roots of E. purpurea and stem segments of A. roxburghii as explants,the standard MS and the national authorized invention patent medium without explosive compounds were used as controls,the effectiveness of 8 brands of powder MS medium on callus induction,adventitious bud regeneration,and rooting of E. purpurea and A. roxburghii was compared.[Result]The three brands of powder MS medium codes M5,M6 and M8 were more effective in callus induction of E. purpurea,and no signifi-cant difference compared with the two controls (CK1 and CK2 ),whereas the other five brands were significantly worse than the control. The re-sults of the experiment on inducing adventitious bud of E. Purpurea showed that five brands medium codes M1,M2,M3,M4 and M7 failed to induce adventitious buds either by using leaves or petioles as explants in E. Purpurea,while the M5 medium not only successfully induced but also obtained more adventitious buds than the control. On the other hand,the comparative experiment of A. Roxburghii tissue culture showed that all 10 mediums could successfully induce to obtain adventitious buds,and the induction rate of adventitious buds in M4 was higher than the control,reaching 83.5％. The results of inducing adventitious root in E. Purpurea showed that only the medium codes M5 and M8 could success-fully induce adventitious roots from adventitious buds as well as the two controls,but the number and length of adventitious roots induced by M5 medium were lower than the two controls. In the experiment of inducing lateral bud rooting of A. Roxburghii,all 8 brands medium could suc-cessfully induce adventitial roots,and the number and length of adventitious roots induced by M2 were significantly higher than the control.[Conclusion]The effects of 8 brands of powder MS medium were significant different in the tissue culture of E. Purpurea and A. Roxburghii,and except for the medium codes M5,M6,and M8,other brands of powder MS medium are not suitable for tissue culture of E. Purpurea. How-ever,except for M4,all 7 other brands of powder MS medium are suitable for A. Roxburghi.
Powder MS mediumE. purpureaA. roxburghiiAdventitious budAdventitious roots
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