Determination of Five Plant Growth Regulator in Red Jujube by QuEChERS Purification High-performance Liquid Chromatography Tandem Mass Spectrometry
[Objective]A method was developed for the simultaneous determination of 5 plant growth regulators (PGRs) in red jujube by using 1％ formic acid-acetonitrile extraction and QuEChERS cleanup with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) detection.[Method]The effects of different extraction reagents,purification materials,etc. on the extraction and purification efficiency were compared. Data were collected in multiple response monitoring (MRM) mode in ESI source positive/negative ion mode for qualitative screening and quantitative analysis.[Result]The linear relationship of the five plant growth regulators was good within the mass con-centration range of 1-50 ng/mL (R2>0.999),the detection and quantification limits were 0.1-0.2 and 0.3-0.6μg/kg,respectively. The re-covery rates of three different addition levels of blank red jujube were 82.5％-106.1％,the relative standard deviation (RSD) was 1.0％-8.4％.Through the detection of actual samples in the market,the level of PGRs in red jujube could be accurately quantified.[Conclusion]The method is simple,rapid,accurate and reliable,and suitable for the rapid determination of five plant growth regulators in jujube.
万方数据
维普
