Screening and Identification of Chitin Degrading Bacteria in Seawater and Optimizing Culture Conditions
[Objective]To obtain high-yield chitinase strains from seawater and optimize culture conditions.[Method]The seawater from Jin-sha Bay of Zhanjiang City was collected as samples,chitinase producing strains were isolated using colloidal chitin as the only carbon source,the enzyme activity was measured by DNS method and molecular identification;single factor experiment and orthogonal optimization were used to optimize the optimal conditions for enzyme production.[Result]Six strains of chitinase-degrading bacteria were screened.The strain with the strongest chitinase activity was Z-4.The strain was identified as Stenotrophomonas maltophilia by morphological observation and 16S rDNA se-quence analysis.The optimum conditions for producing enzyme of strain Z-4 were obtained as follows:soluble starch addition of 1.5%,peptone addition of 2.5%,KH2 PO4 addition of 0.04%;at 30℃,pH 7.0,inoculation amount of 2%,160 r/min and 72 hours of cultivation,the enzyme activity reached 0.452 U/mL,which was 5 times higher than before optimization.[Conclusion]The research results provide an important refer-ence for the effective use of chitin in the ocean to improve the environment.
SeawaterBacteriaChitinaseOptimization of culture conditionsScreening and identification
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