Horizontal Infiltration and Mass Transfer Effects of Plant Fibers on Nicotinic Acid and Vitamin B6 in MS Culture Medium
In order to screen the fibers used in Curtain-liking Anchoring Culture Bioreactor,a"Fiber Horizontal Infiltration Mass Transfer Sim-ulation Device"was created by simulating the process of the reactor to infiltrate and transfer the culture solution to the callus cells through the fiber silk.In combination with the improved simultaneous detection methods for nicotinic acid,vitamin B6,and vitamin B1 HPLC,the mobile phase elution system used sodium heptanesulfonate solution:acetonitrile=90∶10(V∶V)equal degree elution,etc.The effects of mass transfered by horizontal infiltration in 16 plant fiber bundles on nicotinic acid,vitamin B6 and vitamin B1 of MS medium were studied.The results showed that the horizontal infiltration mass transfer in 16 plant fiber bundles had the effect of increasing or decreasing the concentration of nicotinic acid and vitamin B6 of MS culture medium,but the effect duration was not more than 84 min,and there was a significant difference between species.The effect of herb stalk and wood fiber was weaker than that of stem bark and bamboo wood fiber.The concentration of niacin in bamboo fiber bundles of Bambusa emeiensis and Bambusa textilis showed significant changes,ranging from 0.02 to 2.58 μg/mL.The highest increase for niacin concentration was observed in Boehmeria nivea stem bark fiber bundles,reaching 3.17 μg/mL,which was more than five times the origi-nal concentration and lasted for 72 minutes.The vitamin B6 concentration in the mass transfer fluid of Bambusa textilis and Phyllostachys hetero-cycla fiber bundle decreased to zero,and the concentration of vitamins B6 in the mass transfer fluid through Boehmeria nivea stem bark fiber bundle increased to two times as much as the original concentration,while the concentration of three vitamins in other fiber bundles increased or decreased slightly.Although plant fiber bundle can lead to an increase or decrease of the three vitamins concentration of the mass transfer flu-id,the duration of the effect is only within 84 minutes after the fiber bundle were infiltrated and fulled adequately by the medium fluid.
Plant fiberMS culture solutionNicotinic acidVitamin B6Infiltration mass transfer effectHPLC
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