High Quality Fruit Radish Germplasm Screening Based on Analysis of Glucosinolate Species and Content
In this study,12 radish materials with excellent quality were selected from 78 radish materials by sensory evaluation of spiciness,sweetness,texture,hollow,smoothness and scar.Subsequently,LC-MS was used to identify and analyze the components and contents of glu-cosinolate in the fleshy roots of 12 radish materials.Finally,the correlation between total glucosinolate and single glucosinolate was studied by principal component analysis.The results showed that the 12 radish materials selected by sensory evaluation included 2 long-white materials,3 round-white materials,3 long-red materials and 4 green-skin materials.A total of 12 glucosides were identified in the tested materials,inclu-ding 8 aliphatic glucosides(GRA,GIB,GRH,GRE,GER,GBN,PRO,GAL)and 4 indole glucosides(GBR,NGBS,4HGBS,4MGBS).The highest content of total glucosinolates was F1005(22.947 μmol/g),and the lowest content was W1063(3.518 μmol/g)in the tested ma-terials.In terms of composition,the main aliphatic glucosinolate was GRH,and the main indole glucosinolate was NGBS.In addition,aliphatic glucosinolate accounted for 95.78%-99.87%of the total glucosinolate content and indole glucosinolate accounted for 0.16%-4.22%of the to-tal glucosinolate content in the tested materials.The highest glucosinol content of all materials was GRH,which accounted for 61.91%-93.54%of the total glucosinol content.The total glucosinol content was positively correlated with GRH and GRE content,and nega-tively correlated with NGBS content,and there were different degrees of correlation between single glucosinol content.In summary,three mate-rials with high content of sulforaphane precursor glucoside GRA(F1060,F1078 and W1044)were screened in this study,and three materials with low content of glucoside GRH,the main spicy substance,were W1063,F1030 and W1044,respectively.The results will provide the ba-sis for cultivating new varieties of high quality radish.
Fruit radishSensory evaluationGlucosinolateComponent contentLC-MS
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