摘要
为研究新型鹅星状病毒(Novel goose astroviruses,N-GAstV)的遗传进化特征,获得了鹅星状病毒安徽分离株AstV/AHHX的全长基因,为7 251 nt.测序分析显示:AstV/AHHX具有典型的新型鹅星状病毒基因组特征.AstV/AHHX与分离株GDZJ37 以及山东分离株G538和G576的遗传距离较近;同时与毒株GDZJ37、G538和G576的ORF2氨基酸序列同源性达100%,ORF1a和ORF1b的同源性也有99.6%以上,推测它们由同一毒株演化而来.ORF2氨基酸序列位点分析显示有12 高变异位点,分别是第36(Y/S/H)、60(V/I)、224(T/A)、228(A/S/T)、229(P/Q)、289(T/A)、376(T/A)、456(D/E)、540(Q/L)、608(S/T)、610(E/D/G)、614(N/A/T)位氨基酸.其中第224、228、229、608、614位的氨基酸突变可能导致病毒蛋白质的构象及功能发生变化,进而改变病毒的致病能力.
Abstract
In order to study the genetic and evolutionary characteristics of a novel goose astrovirus(N-GAstV),the full-length genes of Anhui strain AstV/AHHX were cloned in this study,and it was 7 251 nt.Sequencing analysis showed that AstV/AHHX had typical characteristics of N-GAstV.The phylogenetic tree showed that AstV/AHHX was relatively close to GDZJ37 isolates from China,G538 and G576 isolates from Shandong Province in recent years.The homology of ORF2 with GDZJ37,G538 and G576 was 100%,and the homology of ORF1a and ORF1b was more than 99.6%.Presumably they evolved from the same strain.Amino acid sequence analysis of ORF2 revealed 12 highly variable sites.They were the amino acid sites,36(Y/S/H),60(V/I),224(T/A),228(A/S/T),229(P/Q),289(T/A),376(T/A),456(D/E),540(Q/L),608(S/T),610(E/D/G),614(N/A/T).Amino acid mutations at position 224,228,229,608 and 614 may lead to changes in the confor-mation and function of viral proteins,then the pathogenic ability of the virus was altered.
基金项目
安徽省科技重大专项(202003a06020012)
安徽省家禽产业技术体系项目(AHCYJSTX-06)
国家科技期刊平台
NSTL
万方数据