摘要
通过盆栽控水试验,研究自然干旱胁迫下3个茶树品种的叶绿素相对含量、抗氧化酶活性和渗透调节物质含量的变化规律,并运用隶属函数法综合评价3个茶树品种的抗旱性.结果表明:与CK相比,干旱胁迫显著提高了3个茶树品种幼苗叶片中的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性以及丙二醛(MDA)、可溶性蛋白(SP)、可溶性糖(SS)和脯氨酸(Pro)含量,显著降低了叶绿素相对含量.随着干旱胁迫时间延长至 15 d,3 个茶树品种叶片中叶绿素相对含量均呈下降趋势,MDA含量先上升后下降;SOD、POD、CAT活性先快速升高,在胁迫11d后开始趋于平缓;SP、SS和Pro含量均呈显著上升趋势.此外,隶属函数法综合分析结果显示,3个茶树品种的抗旱性表现为板塘C>中茶108>王丰瑞-6号.
Abstract
A potted water control experiment was conduct to study the changes in chlorophyll relative content,antioxidant enzyme activity,and osmotic adjustment substance content of three tea varieties under drought stress,and comprehensively evaluated the drought resistance of three tea varieties by membership function method.The results showed that compared with CK,drought stress significantly increased the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and the contents of malondialdehyde(MDA),soluble protein,soluble sugar and proline in the seedlings leaves of three tea varieties,while significantly reduced the relative content of chlorophyll.With the extension of drought stress time to 15 days,chlorophyll relative content in the leaves of three tea varieties showed a decreased trend,and the malondialde-hyde(MDA)content increased and then decreased;the activities of SOD,POD,and CAT increased rapidly,and tend to stable after 11 days of stress;the contents of soluble protein,soluble sugar,and proline showed a significant increased trend.In addition,the results of membership function method showed that the drought resistance of three tea varieties was ranked as follows:Bantang C>Zhongcha 108>Wangfengrui-6.
基金项目
广西农业科学院基本科研业务专项(桂农科2021JM129)
广西农业科学院基本科研业务专项(桂农科2023YM23)
广西农业科学院基本科研业务专项(桂农科2022YM32)
广西农业科学院基本科研业务专项(桂农科2023YM47)