天目地黄4个变异株形态观测及倍性鉴定
Morphological Observation and Ploidy Identification of 4 Mutant Strains of Rehmannia chingii
宋利娜 1张华丽 1衣彩洁 1赵世伟 1冯慧 1弓传伟 1孙丽萍1
作者信息
- 1. 绿化植物育种北京市重点实验室/北京市园林绿化科学研究院,北京 100102
- 折叠
摘要
在连续开展3年多的地黄属植物收集选育工作过程中,发现了4株表观形态有所变化的不同变异株,编号分别为T-1、T-2、T-3、T-4,对其形态指标进行测量统计及数据分析后发现,与天目地黄相比形态差异明显.具体差异表现为:T-1主茎明显短缩;T-2 柱头外露于花瓣,叶片长宽比变小;T-3柱头外漏于花瓣,主茎明显短缩;T-4单叶叶面积增大近3 倍,主茎明显短缩近无.为明确变异株染色体倍性,用流式细胞仪进行倍性检测分析,结果显示T-1、T-2、T-3峰值均与原株一致,鉴定为2倍体,T-4峰值约为原株的2倍,鉴定为4倍体.
Abstract
In the process of collecting and breeding Rehmannia for more than three years,four single plants with apparent morphological varia-tion were found,numbered as T-1,T-2,T-3 and T-4,respectively.The plant morphological indexes were measured and the results showed that the differences of morphological indexes were obvious compared with the original species.The main stem of T-1 was obviously shortened.The stigma of T-2 was exposed to the petals and the length-to-width ratio of the leaves was smaller.The stigma of T-3 was exposed to the petals and the main stem was obviously shortened.The single leaf area of T-4 was increased by nearly 3 times and the main stem was significantly short-ened.In order to clarify the chromosome ploidy of the mutant plants,the flow cytometry was used for ploidy identification.The results showed that T-1,T-2 and T-3 were identified as diploid,but T-4 was identified as tetraploid.
关键词
天目地黄/地黄属/变异单株/柱头外露/倍性检测Key words
Rehmannia chingii/Rehmannia/Variants/Stigma exsertion/Ploidy detection引用本文复制引用
基金项目
北京市公园管理中心科技课题(ZX2021)
出版年
2024
国家科技期刊平台
NSTL
万方数据