Effects of Soaking Seed with Five Exogenous Substances on Seed Germination of Rhamnus parvifolia
[Objective]To study the effect of soaking seeds with exogenous substances on the germination of Rhamnus parvifolia seeds.[Meth-od]Rhamnus parvifolia seeds were soaked for 12 h with five kinds of exogenous substances,including gibberellin acid 3(GA3),6-benzylamin-opurine(6-BA),chitosan oligosaccharide(COS),indoleacetic acid(IAA)and naphthylacetic acid(NAA),and then sowed.[Result]Rham-nus parvifolia seeds began to germinate after 11 days of sowing,and lasted about 40 days.With the rise of GA3 concentration(0.5-3.5 mmol/L),germination increased.Germination rate(GR),germination energy(GE)and germination index(GI)of the treatment with 3.5 mmol/L GA3 were 31.1%,12.1%and 0.33 seeds/d,which were significantly higher than those of group in distilled water(14.4%,3.3%and 0.16 seeds/d)(P＜0.01).0.5 mmol/L 6-BA was quite good.GR,GE and GI of it were 71.1%,17.8%and 0.8 seeds/d,respectively,which presented significantly higher than those of control group(P＜0.01),and germination time(GT)was earlier 2.6 days than the control group.Seeds soaked with 1.5-3.5 mmol/L 6-BA had little effect.Seeds soaked with 0.025%-0.150%COS could accelerate germination.The treatment with 0.100%COS was the best,of which GR,GE and GI were 84.4%,22.2%and 1.28 seeds/d,and presented significantly higher than those of control group(P＜0.01).The treatment with 0.150%COS was the second,of which GR,GE and GI were higher than those of control group(P＜0.01 or P＜0.05).GT of the two treatments was earlier 6-8 days than the control group.Seeds soaked with 0.75-3.00 mmol/L IAA and 0.2-0.8 mmol/L NAA inhibited seed germination.The partial correlation analysis of four indicators,including GR,GE GI and GT,showed that there was a promoting relationship among the first three indexes,while they all had a negative correlation with GT.The key to shortening GT was improving GR,GE and GI.[Conclusion]0.100%COS and 0.5 mmol/L 6-BA were recommended for seed soaking in Rhamnus parvifolia seed-lings rearing.
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