Biological Characteristics of Alternaria alternate Causing Walnut Branch Blight in Longnan City,Gansu Province
The effects of temperature,carbon source,nitrogen source,light,medium and pH on mycelial growth,sporulation and spore ger-mination rate were studied.The results showed that pH 7 and total darkness were conducive to the mycelial growth,sporulation and spore ger-mination of Alternaria alternata;25℃was optimum to mycelial growth,25-30℃was optimum to sporulation,and 20-30℃was optimum to spore germination;the optimum carbon source for mycelial growth was soluble starch,the nitrogen source was yeast extract,KNO3 and pep-tone,the optimum carbon source and nitrogen source for sporulation were sorbitol and KNO3,respectively,and the optimum carbon source and nitrogen source for spore germination were lactose and NH4 NO3,respectively;PSA,OA and PDA medium were suitable for mycelial growth,LB medium was suitable for sporulation,and OA medium was suitable for spore germination.The results provide a theoretical basis for clarif-ying the occurrence rules of walnut branch blight,and also provide a reference for the comprehensive control measures.
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