Diagnosis and Analysis of a Case of Mixed Infection ALV and MDV from Home-kept Chicken
[Objective]To identify samples of suspected tumor disease from a local chicken farm in Anhui Province.[Method]The chicken liv-er was collected and the virus DNA was extracted.The primers were designed according to the sequence of ALV and MDV that GenBank had registered.The ALV env and MDV meq gene were detected by PCR.The amplified sequence was sequenced and analyzed by evolutionary tree and nucleotide homology.[Result]Detected bands of ALV and MD,and sequence alignment results showed that ALV belongs to subgroup J,while MD is a strong wild virus strain.[Conclusion]This study provided reference data for understanding the mixed infection situation of ALV and MDV in the area.
万方数据
维普
