Study on Chromosome Composition of Hybrid Triploid Loach ( Misgurnus anguillicaudatus) Fin Cell Line
In this study,a natural tetraploid loach (4n=100) was crossed with a diploid loach (2n=50) thus creating the different offspring hybrid combinations (4n♀×2n ♂,2n♀×4n ♂).Chromosome samples of hybrid triploid loach fin cell passed for 20 times were prepared via conventional cold drop method.The chromosome number and karyotype were determined via Giemsa staining;Banding analysis were conducted via Ag-NORs and CMA3/DA/DAPI staining.The results showed that the metaphase chromosome number of the fin cells of the hybrid triploid loach was 3n=75,and the karyotype formula was 15m+6sm+54t,NF=96;three silver staining signal points were observed in the chromosome metaphase of hybrid triploid loach fin cells,located at the end region of the first pair of central centromeric chromosomes ( M1);there were three CMA3 signal points in the chromosome metaphase of hybrid triploid loach fin cells,which were located at the region of chromosomes (M1).The study showed that the chromosome composition of hybrid triploid loach fin cells remained unchanged after 20 times of cell culture passage,and the chromosome number,karyotype and band type were consistent with other somatic chromosomes of triploid loach. The results laid the foundation for the cytogenetics of hybrid triploid loach and provided a theoretical basis for cytogenetics of triploid loaches.
万方数据
维普
