The factors that affected callus induction,multiplication and organ differentiation of garlic were explored and the high efficient re-generation system was established. The hormone concentration and pH value of the culture medium suitable for garlic callus induction and pro-liferation were selected:2 mg/L NAA,2 mg/L 6-BA,1 mg/L 2,4-D and the pH of the medium was 5.8. The hormone concentration and pH value of the culture medium suitable for garlic callus organ differentiation were selected. The optimal medium hormone concentration and pH value for callus differentiation of Cangshan garlic were 1 mg/L NAA,3 mg/L 6-BA and the pH of the medium was 5.8. The optimal medium concentration and pH value for callus differentiation of Soviet garlic were 2 mg/L NAA,2 mg/L 6-BA and the pH of the medium was 6.1. The optimal medium concentration and pH value for callus rooting and differentiation of two varieties are 1 mg/L NAA and pH 5.8. Research has also shown that high concentrations of 2,4-D were beneficial for the induction of garlic callus,but not for its differentiation.
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