[Objective]To identify the antitumor endophytic bacterium HDSW of Taxus chinensis var.mairei and preliminarily study its antitu-mor mechanism.[Method]The strain HDSW was identified based on 16S rDNA sequencing. The inhibitory activity of the organic solvent ex-tract of HDSW fermentation broth on gastric cancer cell line MGC-803 was detected by MTT assay,plate cell colony formation assay,cell adhe-sion assay,scratch assay,and flow cytometry.The molecular mechanism was explored by real-time PCR.[Result]The strain HDSW was identi-fied as Bacillus pumilus.The ethyl acetate extract of the fermentation broth could effectively reduce the survival rate,weaken the adhesion capa-bility,inhibit migration capability,and induce cell apoptosis of the gastric cells MGC-803.The ethyl acetate extract from the fermentation broth of strain HDSW could significantly enhance the expression of pro-apoptotic genes p53,Bax and Caspase 3,and inhibit the expression of anti-ap-optotic gene Bcl-2.[Conclusion]The endophytic Bacillus HDSW from Taxus chinensis var.mairei can produce secondary metabolites with sig-nificant anti-gastric cancer cell MGC-803 activity,which can be extracted by ethyl acetate,and possess potential development value.
关键词
南方红豆杉/内生菌/抗肿瘤活性/细胞增殖/细胞迁移
Key words
Taxus chinensis var.mairei Cheng et L.K.Fu/Endophytic bacteria/Antitumor/Cell proliferation/Cell migration
维普
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