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一株南方红豆杉内生菌HDSW的抗肿瘤活性研究

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[目的]鉴定南方红豆杉抗肿瘤内生菌HDSW,并初步研究其抗肿瘤机制.[方法]基于16S rDNA测序鉴定菌株HDSW的种属,通过MTT试验、平板细胞集落形成试验、细胞黏附试验、划痕试验和流式细胞术检测HDSW发酵液的有机溶剂提取物对胃癌细胞MGC-803的抑制活性,利用实时定量PCR方法探索其抗肿瘤的分子机制.[结果]菌株HDSW被鉴定为芽孢杆菌属(Bacillus pumilus),其发酵液的乙酸乙酯提取物作用于胃癌细胞MGC-803可有效降低其存活率,减弱其黏附能力,抑制迁移能力,诱导细胞凋亡.菌株HDSW发酵液的乙酸乙酯提取物可显著增强促细胞凋亡基因p53、Bax和Caspase 3的表达水平,抑制细胞凋亡拮抗基因Bcl-2的表达.[结论]南方红豆杉内生菌HDSW可产生具有显著抗胃癌细胞MGC-803活性的次级代谢产物,且乙酸乙酯可将其提取出来,具有潜在的开发价值.
Antitumor Activity of an Endophytic Bacteria HDSW from Taxus chinensis var.mairei Cheng et L.K.Fu
[Objective]To identify the antitumor endophytic bacterium HDSW of Taxus chinensis var.mairei and preliminarily study its antitu-mor mechanism.[Method]The strain HDSW was identified based on 16S rDNA sequencing. The inhibitory activity of the organic solvent ex-tract of HDSW fermentation broth on gastric cancer cell line MGC-803 was detected by MTT assay,plate cell colony formation assay,cell adhe-sion assay,scratch assay,and flow cytometry.The molecular mechanism was explored by real-time PCR.[Result]The strain HDSW was identi-fied as Bacillus pumilus.The ethyl acetate extract of the fermentation broth could effectively reduce the survival rate,weaken the adhesion capa-bility,inhibit migration capability,and induce cell apoptosis of the gastric cells MGC-803.The ethyl acetate extract from the fermentation broth of strain HDSW could significantly enhance the expression of pro-apoptotic genes p53,Bax and Caspase 3,and inhibit the expression of anti-ap-optotic gene Bcl-2.[Conclusion]The endophytic Bacillus HDSW from Taxus chinensis var.mairei can produce secondary metabolites with sig-nificant anti-gastric cancer cell MGC-803 activity,which can be extracted by ethyl acetate,and possess potential development value.

Taxus chinensis var.mairei Cheng et L.K.FuEndophytic bacteriaAntitumorCell proliferationCell migration

李平、胡建燃、郭春燕、武玉珍

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晋中学院生物科学与技术系,山西晋中 030619

南方红豆杉 内生菌 抗肿瘤活性 细胞增殖 细胞迁移

山西省基础研究计划(自由探索类)青年项目山西省基础研究计划(自由探索类)面上项目山西省教育厅高等学校科技创新项目山西省教育厅高等学校科技创新项目晋中学院"百亿工程"天然产物开发利用创新团队培育项目

202203021222292202103021234932022L5012021L487

2024

10.3969/j.issn.0517-6611.2024.19.032

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子：0.413
ISSN：0517-6611
年,卷(期)：2024.52(19)
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