The dominant pathogenic bacteria were isolated and screened by tissue culture method and tieback method from natural pathological changes of capsicum chinensis. The dominant pathogenic bacteria were identified by morphological observation and ITS sequence analysis. The inhibitory effect of an antagonistic bacterium—Bacillus subtilis on the dominant pathogenic bacteria was studied. The results showed that the dominant pathogenic bacteria that caused the pathological changes of capsicum during storage was a fungus strain,which was identified as Bot-rytis cinerea. It was found that Bacillus subtilis had good inhibitory effect on Botrytis cinerea with inhibition rate of 87.8%.
关键词
辣椒/病原菌/分离鉴定/生防菌/拮抗作用
Key words
Pepper/Pathogenic bacteria/Isolation and identification/Biocontrol bacteria/Antagonism