Analysis of the Effect of Organic Fertilizer on Secondary Metabolites in Tobacco Leaves
Fertilizer is one of the important factors affecting the yield and quality of tobacco leaves,among which the application of organic fertilizer can promote the increase of tobacco leaf yield and improve the quality of tobacco leaves.To explore the effect of increasing the appli-cation of organic fertilizers on the metabolism of tobacco leaves,Yunyan 87 was used as the material in this study.After applying three different gradients of carbon-based organic fertilizers at 0,900 and 1500 kg/hm2,and by using the liquid chromatography-mass spectrometry method,the metabolites in tobacco leaves 7 days after topping were subjected to untargeted metabolome analysis.A total of 219 secondary metabolites were identified in tobacco leaves.Through comparative analysis of metabolites,it was found that 14 and 26 metabolites were reduced in the ac-cumulation of 900 and 1500 kg/hm2 respectively compared with the control group;7 metabolites in the two treatments were significantly re-duced,which contained three nicotinic metabolites M780(nornicotine),M1021(nicotine)and M1328(cotinine).Further analysis found that 9 of the 33 differential metabolites had accumulation differences between the two groups with additional application of 900 and 1500 kg/hm2;the rest of the differential metabolites had no significant accumulation differences between the two groups.Correlation network analysis of differential metabolites found that phenylpropane pathway metabolites such as kaempferol were closely related to the accumulation changes of nicotine,and also had decreased trend.The above results showed that increasing the application of organic fertilizers could not only significantly reduce the accumulation of nicotinic metabolites in tobacco leaves,but also reduce the accumulation of metabolites in the phenyl-propanoid pathway in tobacco leaves to a certain extent;there was no significant difference between adding 900 and 1500 kg/hm2 organic fer-tilizer on nicotine.In summary,this study clarified the effects of organic fertilizers on secondary metabolites of tobacco leaves,and provided certain theoretical guidance for the rational application of organic fertilizers in subsequent tobacco leaf production.
万方数据
维普
