To understand the Dendrbium chrysotoxum,Dendrobium moniliforme and Dendrobium thyrsiflorum seed sterile plant rapid propaga-tion techniques,in this paper,three kinds of Dendrobium seeds as the research object,discusses different types of medium,hormone and con-centration ratio of 3 kinds of Dendrobium sterile seed germination,protocorm proliferation and seedling seedling rooting effect.The results showed that the highest germination rate of the 3 Dendrobium seeds was on 1/2MS base culture and 1/2MS medium with 10%potato.Dendrobi-um chrysotoxum and Dendrobium thyrsiflorum were optimized with 1/2MS+10%potato+0.50 mg/L6-BA+0.2 mg/L NAA,while Dendrobium moniliforme was optimized with 1/2MS+10%potato+1.0 mg/L 6-BA+0.2 mg/L NAA.The best rooting and seedling medium of Dendrobium chrysotoxum moniliforme was 1/2MS+0.10 mg/L IBA+10%potato+2.0%sucrose+0.6%AGAR,and the best rooting and seedling medium of Dendrobium thyrsiflorum and Dendrobium moniliforme was 1/2MS+0.50 mg/L IBA+10%potato+2.0%sucrose+0.6%AGAR.
维普
万方数据