The Induction Method of the Bulbil Germination and Rooting by Multifactor Treatment in Lilium lancifolium
To obtain the method of bulbils rapid propagation in Lilium lancifolium,and explore the effects of multi-factor treatment on germi-nation and rooting of Lilium lancifolium bulbils,the effects of cold temperature pretreatment,culture substrate and temperature,as well as the interaction among the three factors on the germination and rooting efficiency of the bulbils were studied by using multiple factor analysis of vari-ance.The results showed that the interaction between cold temperature pretreatment and culture substrate significantly impacted the germina-tion and rooting efficiency of bulbils,the influence on the germination of bulbils was extremely significant,and the influence on the rooting of bulbils was significant.The influence of culture temperature on the germination and rooting of bulbils was extremely significant and significant respectively;the influence of culture substrate on the germination of bulbils was significant.The optimal method of the bulbil reproduction was that bulbils were treated at cold temperature for 14 days and cultured in fermented sawdust at 26℃,which germination rate could reach 98.33%and rooting rate could reach 98.89%after 56 days of culture.These results clarified the importance of different culture factors and their interactions on the propagation of bulbils of L.lancifolium,provided technical support for the rapid propagation of bulbils of L.lancifolium in vitro,and also provided reference for the application of bulbils in the production and cultivation of other plants.
Lilium lancifolium bulbilsLow-temperature pretreatmentCulture substrateMultivariate analysis of varianceGermination effi-ciency