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多因素处理诱导卷丹珠芽发芽和生根研究

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为获得卷丹百合珠芽快繁的方法,探究多因素处理对卷丹珠芽发芽和生根的影响,利用多因素方差分析低温预处理、培养基质和培养温度以及三者之间的互作对卷丹珠芽发芽和生根的影响.结果表明,低温预处理与培养基质之间的互作对卷丹珠芽发芽的影响表现为极显著,对珠芽生根的影响表现为显著,且影响占比最大;培养温度对珠芽发芽和生根的影响分别表现为极显著和显著;培养基质仅对珠芽发芽的影响表现为显著;将珠芽经4℃处理14 d后置于26℃发酵锯末中培养,是诱导卷丹珠芽发芽和生根的最佳方式,培养56 d时发芽率可达98.33%,生根率可达98.89%.研究结果阐明了不同培养因素及其互作对卷丹珠芽繁殖的重要性,为卷丹珠芽的离体快繁提供了技术支持,也为珠芽在其他植物生产栽培过程中的应用提供参考.
The Induction Method of the Bulbil Germination and Rooting by Multifactor Treatment in Lilium lancifolium
To obtain the method of bulbils rapid propagation in Lilium lancifolium,and explore the effects of multi-factor treatment on germi-nation and rooting of Lilium lancifolium bulbils,the effects of cold temperature pretreatment,culture substrate and temperature,as well as the interaction among the three factors on the germination and rooting efficiency of the bulbils were studied by using multiple factor analysis of vari-ance.The results showed that the interaction between cold temperature pretreatment and culture substrate significantly impacted the germina-tion and rooting efficiency of bulbils,the influence on the germination of bulbils was extremely significant,and the influence on the rooting of bulbils was significant.The influence of culture temperature on the germination and rooting of bulbils was extremely significant and significant respectively;the influence of culture substrate on the germination of bulbils was significant.The optimal method of the bulbil reproduction was that bulbils were treated at cold temperature for 14 days and cultured in fermented sawdust at 26℃,which germination rate could reach 98.33%and rooting rate could reach 98.89%after 56 days of culture.These results clarified the importance of different culture factors and their interactions on the propagation of bulbils of L.lancifolium,provided technical support for the rapid propagation of bulbils of L.lancifolium in vitro,and also provided reference for the application of bulbils in the production and cultivation of other plants.

Lilium lancifolium bulbilsLow-temperature pretreatmentCulture substrateMultivariate analysis of varianceGermination effi-ciency

刘萱、马艳玲、张欣洁、龚德林、雷美艳、符勇耀

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重庆三峡学院生物与食品工程学院,重庆 404020

长江师范学院现代农业与生物工程学院,重庆 408100

重庆市药物种植研究所,重庆 408435

卷丹珠芽 低温预处理 培养基质 多因素方差分析 发芽率

2024

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2024.52(21)