[Objective]To analyze the effects of different factors on embryoid formation using unpollinated ovaries of Luffa ssp.cultivar'Xulv No.1'as experimental material.[Method]An L16(215)orthogonal test was set up to analyze the effects of ovary collection time,4℃cold pretreatment,35℃dark heat shock treatment,TDZ hormone,6-BA hormone,ABA hormone,NAA hormone and 2,4-D hormone on embry-oid formation in vitro cultured Luffa ssp.ovaries.[Result]Different factors had different effects on the development process of ovary slices.A-mong them,collecting female flowers that opened on the same day and adding TDZ hormone to MS medium had a promoting effect on embryoid formation in ovary slices.However,2,4-D hormone had an inhibitory effect on embryoid formation in Luffa ssp.ovaries but could promote roo-ting of ovary slices.In addition,the interaction between 4℃cold pretreatment and TDZ hormone and between TDZ hormone and female flow-er collection time also had a significant effect on embryoid formation in Luffa ssp..[Conclusion]The effective way to form embryoids from Luf-fa ssp.ovaries is:collecting ovaries on day of flowering+4℃cold pretreatment+0.04 mg/L TDZ hormone induction in vitro.
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