Cloning and Expression Analysis of Squalene Synthase Gene in Lonicera macranthoides
[Objective]To clone the full-length sequence of the squalene synthase gene and perform bioinformatics and expression pattern anal-ysis from Lonicera macranthoides.[Method]Total RNA was extracted from Lonicera macranthoides,and the full-length cDNA sequence of LmSS gene was cloned using RT-PCR and RACE techniques,bioinformatics analysis was conducted on the gene sequence using relevant soft-ware,the relative expression of the gene in stem,leaf,and different flower period was determined by using Real-time PCR.[Result]The open reading frame(ORF)of LmSS gene was 1 245 bp,encoding 414 amino acids.It belongs to a hydrophilic protein and is located in the cy-toplasm.LmSS gene has a typical polyisoprene synthase active domain,which has high homology with other plant SS genes.The expression of LmSS gene is tissue-specific in different flowering stages and organs of Lonicera macranthoides.[Conclusion]This study successfully cloned the SS gene in Lonicera macranthoides,laying a foundation for further research on its function and providing a research basis for exploring the bio-synthesis and regulatory mechanism of saponins in Lonicera macranthoides.
万方数据
维普
