Isolation,Purification and Identification of Parasitic Fungi in Cicada(Hemiptera:Cicadidae)
In order to understand the types of parasitic fungi in cicadas,we isolated and identified entomophytic fungi in cicadas,then observed their main characteristics,sequenced and analyzed ITS sequences of the strains.Several pathogenic fungi were isolated and purified from the ci-cada infected with fungi,named Y1,Y3,and Y6,and their morphology was observed,ribosomal DNA sequencing and analysis were performed to identify and analyze them.Morphologically,it can be inferred that the colonies of fungi Y1 and Y3 are yellow brown in color,with white hyphae and yellow brown spores.They are round and uniform in size.After ITS sequence alignment and combined with morphological characteristics,they belong to the Scopulariopsis alboflavescen genus of fungi.The color of Y6 colony is white,and the hyphae on PDA medium are short and dense,growing slowly.When observed under a microscope,the hyphae are colorless,and the spores are round or elliptical,with uniform sizes.After ITS sequence alignment and combined with morphological characteristics,it is classified as a Scoporariopsis fungus.
万方数据
维普
