The ornamental plant Calathea'Network',belonging to the Araceae and the Calathea,was the material for this study.We studied the effects of types and concentrations of plant growth regulators,inoculation methods on bud proliferation,different media formulations on roo-ting induction,and different cultivation medium on transplant survival rate.The results showed that the single bud inoculation method had a better effect.The culture medium MS+0.1 mg/L NAA+0.5 mg/L BA was suitable for the shoot proliferation in C.'Network'.After 30 days of cultivation,the induction rate of adventitious buds was 98.89%,and proliferative buds was 3.29.The optimal medium for rooting 1/2MS+0.1 mg/L NAA+0.5 g/L AC could reach transplanting standard in 15-25 days.The peat∶coir=3∶1 substrate was suitable for transplanting the in vitro seedlings of C.'Network'and the survival rate of transplanting was 97.22%.This study successfully established an efficient in vitro propagation system for the industrialization of the plant,providing technical support for its industrial production.
维普
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