Application of Droplet Digital PCR in the Detection of Pseudomonas savastanoi
In order to realize high efficiency and high precision quarantine identification and epidemic monitoring of Pseudomonas savastanoi pv.phaseolicola(Psp),the detection method of Psp by droplet digital PCR(ddPCR)was established.The ddPCR reaction system was conduc-ted with Psp as research object and site-specific recombinases were used as gene target with SSRP-F/SSRP-R/SSRP-P as primers and probe.The results showed that the detection limit of genomic DNA by ddPCR was 0.6 copies/μL.The detection sensitivity of ddPCR could increase two orders of magnitude than convention PCR.Meanwhile,the activity of Psp carried in peas was studied,which improved the epidemic detec-tion rate.
Droplet digital PCRPseudomonas savastanoi pv.phaseolicolaRapid detection
万方数据
维普
