Research on Key Techniques for Tissue Culture of Rhododendron lapponicum
This study used the leaves,stem segments,and bracts of alpine rhododendron varieties'23XXL''5RE'and'6GR'as explants to investigate the effects of basic culture medium,physiological age of explants,and plant growth regulators on callus induction in alpine rhodo-dendron explants.The results showed that the explants of alpine rhododendron could induce callus on both WPM and MS media.During the callus induction stage,adding 2.0-3.0 mg/L TDZ had a good effect on inducing callus.Among them,the callus induced by leaves with a physiological age of 2W had better growth,and the highest callus rate was 100%;at the stage of callus differentiation,the most suitable ratio of callus differentiation hormones was ZT 2.5 mg/L+NAA 0.5 mg/L and TDZ 1.5 mg/L+NAA 1.0 mg/L.Among them,the explant with a physiological age of 1M had better callus differentiation on Z7 medium,with an average number of bud differentiation of 0.92;during the roo-ting stage,the optimal hormone ratio of the culture medium was IBA 0.5 mg/L+NAA 1.0-1.5 mg/L,which were suitable for the three varie-ties of alpine rhododendron.The research results provide a scientific basis for constructing an efficient and stable tissue culture and rapid prop-agation system of alpine rhododendron,and lay a foundation for further realizing the industrialization of alpine rhododendron.
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