The seedlings of mature Ribes maximowiczianum Komarov seed germination under different disinfection treatment were used as ex-plants to study the influence of different plant growth regulators on buds induction,hyperplasia,sound seedling and rhizogenesis.The results showed that 500 mg/L GA3 soaking treatment was conducive to seed germination,and the germination rate could reach 66.7%,and the best disinfection treatment was 75%alcohol for 30s and 0.1%mercuric acid disinfection for 8 min,the contamination rate was 23.3%.The best me-dium for bud induction was MS+2.0 mg/L 6-BA+0.05 mg/L NAA,the proliferation times was 7.5.The best medium for rooting was 1/2MS+2.0 mg/L IBA,the rooting rate was100%,and the average number of rooting shoots was 6.33.The survival rate was 87.5%in the medium of 3∶1∶1 in peat soil∶coir∶perlite.This study established a complete system of tissue culture and rapid propagation for Ribes maximo-wiczianum Komarov seed,which is suitable for the large-scale production and application of Ribes maximowiczianum Komarov seedlings.
Seeds of Ribes maximowiczianum KomarovTissue cultureCluster budsRapid propagation
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