尖叶茶藨子种子无菌苗再生体系的建立

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国家科技期刊平台
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中文摘要：以尖叶茶藨子成熟种子为试材,采用不同处理及消毒方式获得无菌苗,再以幼苗茎段为外植体,研究不同植物生长调节剂对丛芽诱导、增殖、壮苗及生根的影响.结果表明:500 mg/L GA3 浸泡处理有利于种子的萌发,发芽率可达 66.7%,最佳消毒处理为 75%乙醇30 s+0.1%HgCl2 消毒8 min,污染率为23.3%;最佳丛芽诱导培养基为MS+2.0 mg/L 6-BA+0.05 mg/L NAA,增殖倍数为7.5;最佳壮苗培养基为MS+0.1 mg/L 6-BA+0.05 mg/L NAA+50 g马铃薯泥;最佳生根培养基为1/2MS+2.0 mg/L IBA,生根率为100%,平均生根条数为6.33.炼苗后,移栽到泥炭土∶椰糠∶珍珠岩体积比为3∶1∶1的基质中,成活率达87.5%.该研究建立了尖叶茶藨子种子组培快速繁育技术体系,适用于尖叶茶藨子种苗的规模化生产和应用.

外文标题：Establishment of Rapid Propagation for Sterile Seedlings of Ribes maximowiczianum Komarov by Tissue Culture

外文摘要：The seedlings of mature Ribes maximowiczianum Komarov seed germination under different disinfection treatment were used as ex-plants to study the influence of different plant growth regulators on buds induction,hyperplasia,sound seedling and rhizogenesis.The results showed that 500 mg/L GA3 soaking treatment was conducive to seed germination,and the germination rate could reach 66.7%,and the best disinfection treatment was 75%alcohol for 30s and 0.1%mercuric acid disinfection for 8 min,the contamination rate was 23.3%.The best me-dium for bud induction was MS+2.0 mg/L 6-BA+0.05 mg/L NAA,the proliferation times was 7.5.The best medium for rooting was 1/2MS+2.0 mg/L IBA,the rooting rate was100%,and the average number of rooting shoots was 6.33.The survival rate was 87.5%in the medium of 3∶1∶1 in peat soil∶coir∶perlite.This study established a complete system of tissue culture and rapid propagation for Ribes maximo-wiczianum Komarov seed,which is suitable for the large-scale production and application of Ribes maximowiczianum Komarov seedlings.

外文关键词：

Seeds of Ribes maximowiczianum KomarovTissue cultureCluster budsRapid propagation

作者：

林丽霞、林建忠、张雪芹、钟赞华、赖瑞云

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作者单位：

福建省亚热带植物研究所/福建省亚热带植物生理生化重点公共实验室,福建厦门 361006

关键词：

尖叶茶藨子种子组织培养丛生芽快速繁育

出版年：

2024

DOI：