新型植物免疫激活蛋白维大力(VDAL)对烟草花叶病毒抑制作用
Inhibitory Effect of Novel Plant Immune Activating Protein VDAL on Tobacco Mosaic Virus
黑雅娅 1李永亮 2郭琳琳 1胡丽杰 1葛华梅 1王文立 3范俊辉1
作者信息
- 1. 杨凌翔林农业生物科技有限公司,陕西杨凌 712100
- 2. 云南省保山市烟草公司,云南保山 678000
- 3. 中捷四方生物科技股份有限公司,北京 101102
- 折叠
摘要
[目的]明确新型植物免疫激活蛋白维大力(VDAL)对烟草花叶病毒(TMV)的抑制作用.[方法]采用半叶枯斑法和分光光度法,分别测定植物免疫激活蛋白VDAL对TMV的抑制作用和烟草植株过氧化物酶(POD)活性的影响.[结果]VDAL对TMV具有较好的体外钝化作用,当钝化时间为90 min时,枯斑抑制率可达到80.76%;喷药 3 次后抑制率为 85.04%,且过氧化物酶活性较对照增加了1.26倍.[结论]VDAL可能通过对病毒的钝化作用并诱导烟草植株产生防御反应,进而防治烟草花叶病毒病.
Abstract
[Objective]To clarify the inhibitory effect of a new plant immune activating protein(VDAL)on tobacco mosaic virus(TMV).[Method]The inhibition of plant immune activating protein(VDAL)on TMV and the effect on peroxidase(POD)activity in tobacco plants were determined by spot inhibition and spectrophotometry.[Result]VDAL had a good inactivation effect on TMV in vitro.When the inactiva-tion time was 90 min,the inhibition rate of lesions could reach 80.76%.After three times of spraying,the inhibition rate could rise to 85.04%,and the peroxidase activity increased 1.26 times compared with the control.[Conclusion]VDAL may control TMV disease by inactivating the virus and inducing the defense response of tobacco plants.
关键词
烟草花叶病毒/维大力/半叶枯斑法/分光光度法/过氧化物酶Key words
Tobacco mosaic virus/VDAL/Half-leaf spot method/Spectrophotometry/Peroxidase引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据