首页|新型植物免疫激活蛋白维大力(VDAL)对烟草花叶病毒抑制作用

新型植物免疫激活蛋白维大力(VDAL)对烟草花叶病毒抑制作用

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[目的]明确新型植物免疫激活蛋白维大力(VDAL)对烟草花叶病毒(TMV)的抑制作用.[方法]采用半叶枯斑法和分光光度法,分别测定植物免疫激活蛋白VDAL对TMV的抑制作用和烟草植株过氧化物酶(POD)活性的影响.[结果]VDAL对TMV具有较好的体外钝化作用,当钝化时间为90 min时,枯斑抑制率可达到80.76%;喷药 3 次后抑制率为 85.04%,且过氧化物酶活性较对照增加了1.26倍.[结论]VDAL可能通过对病毒的钝化作用并诱导烟草植株产生防御反应,进而防治烟草花叶病毒病.
Inhibitory Effect of Novel Plant Immune Activating Protein VDAL on Tobacco Mosaic Virus
[Objective]To clarify the inhibitory effect of a new plant immune activating protein(VDAL)on tobacco mosaic virus(TMV).[Method]The inhibition of plant immune activating protein(VDAL)on TMV and the effect on peroxidase(POD)activity in tobacco plants were determined by spot inhibition and spectrophotometry.[Result]VDAL had a good inactivation effect on TMV in vitro.When the inactiva-tion time was 90 min,the inhibition rate of lesions could reach 80.76%.After three times of spraying,the inhibition rate could rise to 85.04%,and the peroxidase activity increased 1.26 times compared with the control.[Conclusion]VDAL may control TMV disease by inactivating the virus and inducing the defense response of tobacco plants.

Tobacco mosaic virusVDALHalf-leaf spot methodSpectrophotometryPeroxidase

黑雅娅、李永亮、郭琳琳、胡丽杰、葛华梅、王文立、范俊辉

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杨凌翔林农业生物科技有限公司,陕西杨凌 712100

云南省保山市烟草公司,云南保山 678000

中捷四方生物科技股份有限公司,北京 101102

烟草花叶病毒 维大力 半叶枯斑法 分光光度法 过氧化物酶

2024

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2024.52(22)