[Objective]A method for simultaneous determination of β-sitosterol,stigmasterol,campesterol and brassicasterol in edible oil was established by gas chromatography-mass(GC-MS).[Method]Sample was saponified by potassium hydroxide-ethanol solution,n-hexane extrac-tion,concentration,derivatization,and then diluted with n-hexane.GC-MS was used to select ion scanning mode(SIM)and external standard method was used for quantification.[Result]There were good linear relationships for β-sitosterol,stigmasterol,brassicasterol in 5-500 μg/mL,and campesterol in 2.5-250.0 μg/mL,respectively.Linear correlation coefficients(R2)were greater than 0.999.Limits of detection were 0.05-0.10 mg/kg and limits of quantification were 0.1-0.3 mg/kg,respectively.Adding standard recovery rate was 80.5%-107.8%,relative standard deviation(RSD)was 2.3%-9.8%.[Conclusion]This method is easy to operate,efficient,highly sensitive,and has good repeatability,which is suitable for the determination of β-sitosterol,stigmasterol,campesterol and brassicasterol contents in edible vegetable oil samples.
Gas chromatography-mass spectrometry(GC-MS)Edible vegetable oilPhytosterolContent determination
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