气相色谱-质谱法测定食用植物油中多种植物甾醇含量
Determination of Various Phytosterols in Edible Vegetable Oils by Gas Chromatography-mass Spectrometry
黄何何1
作者信息
- 1. 福建省产品质量检验研究院,福建福州 350002
- 折叠
摘要
[目的]建立气相色谱-质谱法(GC-MS)同时测定食用油中β-谷甾醇、豆甾醇、菜油甾醇、菜籽甾醇含量的方法.[方法]样品选用氢氧化钾-乙醇皂化、正己烷萃取、浓缩、衍生化后以正己烷定容,采用GC-MS选择离子扫描模式(SIM),外标法定量.[结果]β-谷甾醇、豆甾醇、菜籽甾醇的线性范围为5～500 μg/mL,菜油甾醇的线性范围为2.5～250.0 μg/mL,线性相关系数(R2)均大于0.999,检出限为0.05～0.10 mg/kg,定量限为0.1～0.3 mg/kg,加标回收率为80.5%～107.8%,相对标准偏差(RSD)为2.3%～9.8%.[结论]该方法操作简单、高效、灵敏度高、重复性好,适用于食用植物油样品中β-谷甾醇、豆甾醇、菜油甾醇、菜籽甾醇含量的测定.
Abstract
[Objective]A method for simultaneous determination of β-sitosterol,stigmasterol,campesterol and brassicasterol in edible oil was established by gas chromatography-mass(GC-MS).[Method]Sample was saponified by potassium hydroxide-ethanol solution,n-hexane extrac-tion,concentration,derivatization,and then diluted with n-hexane.GC-MS was used to select ion scanning mode(SIM)and external standard method was used for quantification.[Result]There were good linear relationships for β-sitosterol,stigmasterol,brassicasterol in 5-500 μg/mL,and campesterol in 2.5-250.0 μg/mL,respectively.Linear correlation coefficients(R2)were greater than 0.999.Limits of detection were 0.05-0.10 mg/kg and limits of quantification were 0.1-0.3 mg/kg,respectively.Adding standard recovery rate was 80.5%-107.8%,relative standard deviation(RSD)was 2.3%-9.8%.[Conclusion]This method is easy to operate,efficient,highly sensitive,and has good repeatability,which is suitable for the determination of β-sitosterol,stigmasterol,campesterol and brassicasterol contents in edible vegetable oil samples.
关键词
气相色谱-质谱法/食用植物油/植物甾醇/含量测定Key words
Gas chromatography-mass spectrometry(GC-MS)/Edible vegetable oil/Phytosterol/Content determination引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据