首页|活性炭对极大节旋藻来源藻蓝蛋白和蛋白质的吸附性能和机制研究

活性炭对极大节旋藻来源藻蓝蛋白和蛋白质的吸附性能和机制研究

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通过等温吸附、吸附动力学和吸附热力学试验,揭示活性炭对C-PC和总蛋白质的吸附特性和作用机制.结果显示:同样条件下,活性炭去除杂蛋白质的效率更高,经过活性炭纯化后,C-PC占总蛋白质的比例从31.2%增加到51.5%;活性炭对C-PC和总蛋白质的最大吸附量分别为10.5和88.5 mg/g;活性炭对C-PC的吸附为单分子层化学吸附,符合Langmuir等温吸附模型,而对总蛋白质的吸附为多层级的吸附,符合Freundlich等温线模型.
Study on Adsorption Capacity and Mechanism of Activated Charcoal on Arthrospira maxima-derived C-phycocyanin and Proteins
The isotherms,adsorption kinetics,and thermodynamics analyses were employed to elucidate the adsorption properties and mecha-nisms of action of activated charcoal on C-PC and total proteins.The results revealed that:under the same conditions,activated charcoal was more efficient in removing heteroproteins,and after purification with activated charcoal,the proportion of C-PC to the total protein increased from 31.2%to 51.5%;the maximum adsorption of C-PC and total protein by activated charcoal was 10.5 mg/g and 88.5 mg/g,respectively;the adsorption of C-PC by activated charcoal was a monomolecular layer chemisorption,consistent with the Langmuir isothermal adsorption model,whereas the adsorption of total proteins of activated charcoal was a multilayer adsorption,consistent with the Freundlich isotherm model.

SpirulinaC-phycocyanin(C-PC)Protein extractionActivated charcoalAdsorption isotherm models

白仁奥

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汕头职业技术学院建设生态学院,广东汕头 515078

螺旋藻 藻蓝蛋白 蛋白质提取 活性炭 吸附等温曲线

2024

安徽农业科学
安徽省农业科学院

安徽农业科学

影响因子:0.413
ISSN:0517-6611
年,卷(期):2024.52(23)