活性炭对极大节旋藻来源藻蓝蛋白和蛋白质的吸附性能和机制研究
Study on Adsorption Capacity and Mechanism of Activated Charcoal on Arthrospira maxima-derived C-phycocyanin and Proteins
白仁奥1
作者信息
- 1. 汕头职业技术学院建设生态学院,广东汕头 515078
- 折叠
摘要
通过等温吸附、吸附动力学和吸附热力学试验,揭示活性炭对C-PC和总蛋白质的吸附特性和作用机制.结果显示:同样条件下,活性炭去除杂蛋白质的效率更高,经过活性炭纯化后,C-PC占总蛋白质的比例从31.2%增加到51.5%;活性炭对C-PC和总蛋白质的最大吸附量分别为10.5和88.5 mg/g;活性炭对C-PC的吸附为单分子层化学吸附,符合Langmuir等温吸附模型,而对总蛋白质的吸附为多层级的吸附,符合Freundlich等温线模型.
Abstract
The isotherms,adsorption kinetics,and thermodynamics analyses were employed to elucidate the adsorption properties and mecha-nisms of action of activated charcoal on C-PC and total proteins.The results revealed that:under the same conditions,activated charcoal was more efficient in removing heteroproteins,and after purification with activated charcoal,the proportion of C-PC to the total protein increased from 31.2%to 51.5%;the maximum adsorption of C-PC and total protein by activated charcoal was 10.5 mg/g and 88.5 mg/g,respectively;the adsorption of C-PC by activated charcoal was a monomolecular layer chemisorption,consistent with the Langmuir isothermal adsorption model,whereas the adsorption of total proteins of activated charcoal was a multilayer adsorption,consistent with the Freundlich isotherm model.
关键词
螺旋藻/藻蓝蛋白/蛋白质提取/活性炭/吸附等温曲线Key words
Spirulina/C-phycocyanin(C-PC)/Protein extraction/Activated charcoal/Adsorption isotherm models引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据