Effects of Exogenous Active Substances on Physiological and Biochemical Indexes and Yield Quality of Upper Tobacco Leaves
In order to screen exogenous active substances that can effectively regulate the development and maturation process of tobacco leav-es,to improve the maturity tolerance of upper tobacco leaves,to promote their full maturity,to improve quality,and to provide technical basis for high-quality tobacco production in upper part of Guiyang City,we used random block design and adopted 4 treatments of CK,spraying wa-ter,T1(spraying 5 mg/L spermidine),T2(spraying 1 mg/L brassinolide),T3(spraying 40 mg/L methyl jasmonate).Results showed that compared with the CK,different exogenous active substances significantly increased the levels of auxin and cytokinin in upper leaves,decreased the level of abscisic acid,enhanced chlorophyll enzyme activity,increased SPAD value,significantly improved cell membrane stability index,and improved cell membrane stability.It significantly increased the pitch and leaf number of tobacco plants,and significantly enhanced the yield and output value of upper tobacco leaves.Among them,T3 treatment was the highest,which was 19.03%and 23.17%higher than that of the control,respectively.The contents of total sugar,reducing sugar and potassium in T2 and T3 treatments were higher,and the nicotine content in all treatments decreased.The ratio of two sugars in T1 and T3 treatments was greater than 0.9,and the ratio of sugar to alkali in different exoge-nous active substances treatments was in the range of 6.0-10.0,while CK was lower.The ratio of two sugars and the ratio of sugar to alkali in different treatments were the highest in T3 treatment,with values being 0.96 and 9.24,respectively.The ratio of potassium to chlorine in all treatments was greater than 4.0,of which T2 treatment was the highest,with a value of 10.90;the appearance quality and sensory quality of up-per tobacco leaves were improved,among which T2 treatment was the best,and the values were 6.00 and 60.00 points,respectively.In gener-al,spraying 1 mg/L brassinolide T2 treatment could promote the growth of upper tobacco leaves,improve the stability of cell membrane,delay senescence,improve the ventilation and light transmission conditions of tobacco plants,increase the number of leaves,enhance the yield and output value of upper tobacco,increase the content of sugar and potassium,decrease the content of nicotine,significantly increase the ratio of sugar to alkali,improve the appearance quality and sensory quality of tobacco leaves,improve the maturity and identity of tobacco leaves,im-prove the aroma quality and aroma quantity and reduce the irritation.
Exogenous active substanceUpper tobacco leafPhysiological and biochemical indexesYield and quality
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