Transcriptomic Analysis of White Leaf Traits in Triticum aestivum L.at Regreening Stage
[Objective]To decipher the mechanism of leaf color albinism in Triticum aestivum L.white-leaf mutants.[Method]This study con-ducted a comparative transcriptomic analysis of the leaf color change process using normal Triticum aestivum L.and temperature-sensitive white-leaf mutants.The aim was to uncover the regulatory mechanisms governing the formation of photosynthetic pigments in Triticum aestivum L..[Result]The results showed that the number of Differentially Expressed Genes(DGEs)involved in the development of normal Triticum aesti-vum L.leaves was 2.57 times that in the process of white leaf color turning green.DGEs in the normal leaf development process were mainly in-volved in the metabolism of lipids,amino acids,proteins,purines,and inositol,among other biomacromolecules.In contrast,DGEs in the process of leaf color turning from white to green were primarily involved in photosynthesis,respiration,and carbohydrate metabolism.Furthermore,it was found that Triticum aestivum L.possesses all the genes in the known plant photosynthetic pigment metabolic pathways,75 of which were DGEs.These DGEs included all genes of the chlorophyll synthesis pathway and two carotenoid synthesis pathway genes.Their expression patterns can be classified into seven categories(Ⅰ to Ⅶ).These patterns suggest that the biosynthesis of Triticum aestivum L.chlorophyll is subject to feed-back regulation,and that impaired chlorophyll synthesis leads to the formation of white leaves,with TaCAO likely being the main factor affecting chlorophyll synthesis.[Conclusion]These findings provide a theoretical basis for further research into the chlorophyll formation mechanism in Triticum aestivum L..
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